Options

Trajectory Correction +: If checked, tracked particles are re-analyzed using an optimized nearest-neighbor algorithm in order to correct disconnected trajectories. For each trajectory, the algorithm searches for trajectories starting on the following frames matching the specified conditions (distance, angle, acceleration, area, intensity). For each retained pair of trajectories, the algorithm computes a correlation score using the root mean squared of the specified ratios (distance, angle, acceleration, area, intensity). The algorithm then determines the most probable pairs by selecting the ones maximizing the correlation score. The trajectories of the selected pair are then re-connected.

Delta max: The maximum number of frames between the last particle of a trajectory and the first particle of a second trajectory to be re-connected. Default setting is 2.

Distance max: The maximum distance between the last particle of a trajectory and the first particle of a second trajectory to be potentially re-connected. Value is expressed in pixels or in calibrated units. Default setting is 2.

Angle: If checked, the angle ratio between the first trajectory and the second trajectory will be used as a condition. This command is enabled when the tracking mode is ‘Mobile’. Default setting is checked.

Acceleration: If checked, the acceleration ratio between the first trajectory and the second trajectory will be used as a condition. This command is enabled when the tracking mode is ‘Mobile’. Default setting is checked.

Area: If checked, the area ratio between the last particle of the first trajectory and the first particle of the second trajectory will be used as a condition. Default setting is checked.

Intensity: If checked, the intensity ratio between the last particle of the first trajectory and the first particle of the second trajectory will be used as a condition.

Division Correction +: If checked, particle trajectories are re-analyzed in order to correct abnormal division-fusion events. Abnormal division-fusion events occur when a cell appear to divide into two daughter cells that ultimately merge back into a single cell. For each trajectory ending with a division event, the algorithm searches for trajectories starting on the following frames matching the specified conditions (distance, angle, acceleration, area, intensity). If abnormal division-fusion events are detected, the algorithm will correct the abnormal division by merging the boundaries of the two daughter cells. This command is enabled when the tracking mode is ‘Stationary’.

Delta max: The maximum number of frames between the last particle of a trajectory and the first particle of a second trajectory after division to be re-connected. Default setting is 2.

Distance max: The maximum distance between the last particle of a trajectory and the first particle of a second trajectory to be potentially re-connected. Value is expressed in pixels or in calibrated units. Default setting is 2.

Filter +: If checked, particle trajectories are evaluated against the following conditions until the specific attributes are met. If not, the trajectories are rejected.

Lifespan: Trajectories with a lifespan outside the range are rejected. Values are expressed in frame. Default setting is 0-max.

Frame Start: Trajectories starting at a frame outside the range are rejected. Values are expressed in frame. Default setting is 0-max.

Frame End: Trajectories ending at a frame outside the range are rejected. Values are expressed in frame. Default setting is 0-max.

Criteria: Trajectories that do not fit the specified criteria are rejected (see the Type section). Leave empty to turn this attribute off.

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